ABSTRACT
Objective To investigate the effects of PRL-3 shRNA mediated by lentivirus on proliferation, invasion and apoptosis of human colorectal cancer SW480 cells. Methods There were three experimental groups in this study, which included blank control group, negative control group and transfected group. Colorectal cancer SW480 cells were transfected with lentiviral interference vector carrying PRL-3 shRNA to build a stable PRL-3-silencing cell line. The expression of PRL-3 mRNA was detected by real-time quantitative polymerase chain reaction (real-time PCR). Cell proliferation was analyzed by MTT method and colony formation assay.Invasion and migration were measured by Transwell assay and invasion chamber. Apoptosis rate was performed by flow cytometry. Results The stable PRL-3-silencing cell line was successfully constructed.Compared with the blank control group and negative control group,the relative expression levels of PRL-3 mRNA were reduced in transfected group after transfection with PRL-3 shRNA(P<0.05),but there was no significant difference between the blank control group and the negative control group.After transfection with PRL-3 shRNA for 72 h,the proliferation of SW480 cells was significantly lower in transfected group than that of the blank control group and the negative control group,and the proliferation decreased significantly in 120 h(P<0.05).Compared with the blank control group and negative control group, the ability of colony formation was also weakened in the transfected group (P<0.05). Compared with the blank control group and negative control group,the migration and invasion ability were decreased in the transfected group(P<0.05),and the apoptosis rate was increased in the transfected group(P<0.05).Conclusion PRL-3 shRNA can inhibit the expression of PRL-3 and the proliferation, promote the apoptosis of SW480, which indicates that PRL-3 may become a target for colorectal carcinoma treatment.
ABSTRACT
<p><b>OBJECTIVE</b>To summarize the epidemiological and biological features of triple-negative breast cancer (TNBC) and non-triple-negative breast cancer (non-TNBC) to provide reference for devising individualized therapy and making prognostic evaluation.</p><p><b>METHODS</b>The 5-year follow-up data were collected from 231 patients with pathologically established diagnosis of breast cancer treated in the Affiliated Hospital of Ningxia Medical University and Yinchuan People's Hospital between Jan. 2002 and Dec. 2004. The epidemiological and clinicopathological characteristics as well as the recurrence, metastasis and 5-year survival were compared between TNBC group and non-TNBC group.</p><p><b>RESULTS</b>TNBC accounted 17.3% of the total breast cancer cases enrolled in this study. The tumor size and rates of recurrence and metastasis (especially visceral metastasis) were significantly greater in TNBC group than in non-TNBC group (P<0.05). The TNBC patients showed significantly lower 3- and 5-year survival rates than the non-TNBC patients (P<0.05), and TNBC patients with positive lymph nodes in clinical stage II had also a lower 5-year survival (P<0.05). Cox regression model analysis identified the patients' age, primary tumor size, clinical stages and triple-negativity as the independent risk factors for breast cancer.</p><p><b>CONCLUSION</b>Compared to non-TNBC patients, patients with TNBC have higher rates of local recurrence and invasion, visceral metastasis and poorer prognosis, and a lower rate of 5-year survival. The triple negativity represents an independent factor for prognosis evaluation of breast cancer.</p>